Human Calmodulin Methyltransferase: Expression, Activity on Calmodulin, and Hsp90 Dependence
Figure 1
Identification of alternative CaM KMT variants and their expression pattern.
(A) Schematic representation of the new splice variants CaM KMT-1 and CaM KMT-2 that were identified by 5′RACE-PCR, and their positions relative to the known full length CaM KMT and short CaM KMTsh variants. The top of the figure shows the position on chromosome 2 and the ruler of the bases according to genome assembly hg19. The site of the 2p21 deletion is marked by an arrow. (B) Verification of the transcription of the CaM KMT-1 variant by RT-PCR in the lymphoblastoid cells from patients and controls as well as in normal human tissues. The 5′ primer was localized in the newly discovered exon and 3′ primer in 4th exon of CaM KMT. The identity of the products was validated by sequencing. The arrow points to the products of an expected size of 287 bp. -, no cDNA; Lm, lymphoblastoid cells. (C) The sequence of the novel mRNA CaM KMT -1 isoform. Bold bases represent the sequence of the novel exon directly connected to the sequence of the 4th known exon that is marked by an arrow. The open reading frame is underlined and the start codon is shown in uppercase with Kozak consensus sequence shown in bold italic. The stop codon is shown in bold uppercase.