Gene Specific Actions of Thyroid Hormone Receptor Subtypes
Figure 5
T3 Induced Genes are Direct TR Targets.
A. Bar graph representing numbers of T3 induced (upper panels) and repressed (lower panels) genes at 3 hrs in HepG2-TRα and HepG2-TRβ cells that persist with CHX pre-treatment (upper panel, red, lower panel, blue). B. Heat map representing gene expression changes at 3 hrs timepoint in HepG2-TRα and HepG2-TRβ cells with T3, CHX and T3 + CHX. Note that most target genes retain their T3 responses with CHX. Examples of genes with unusual responses are marked by lower case letters: a = stronger T3 responses with TRα, b = amplification of weak T3 responses in the presence of TRβ with CHX, c = selective CHX-dependent gene induction in the presence of TRα, d = selective CHX-dependent gene induction in the presence of TRβ.