The Fate of Acetic Acid during Glucose Co-Metabolism by the Spoilage Yeast Zygosaccharomyces bailii
Figure 2
In vivo 13C-NMR spectra of [2−13C]acetate metabolism and unlabelled glucose by resting cells of Z. bailii, in aerobic conditions.
[2-13C]acetate (83 mM) and unlabelled glucose (63 mM) were added to a cell suspension (4–5 mg dry weight ml-1). 13C-NMR spectra (2.5 min each) were acquired sequentially. An expansion of (A) in the spectral region 25–120 ppm is shown in (B). Symbols: Act-C2 is [2–13C]acetate; Glu-C2, C3 and C4 are the resonances due to carbon atoms 2, 3, and 4, respectively, in glutamate; Glc-C1 (α,β) are the resonances due to carbon 1 in the two anomers of glucose (natural abundance).