SUMOylation Is Required for Glycine-Induced Increases in AMPA Receptor Surface Expression (ChemLTP) in Hippocampal Neurons
Figure 5
Overexpression of SENP-1 diminishes the ChemLTP-mediated increase in SUMO-1 mRNA.
A) Representative images showing effects of ChemLTP in the soma and processes of hippocampal neurons overexpressing SENP-1 C603S (inactive control) or SENP-1 WT (green). Fluorescence in situ hybridization was performed using cRNA probes for SUMO-1 (red). Neurons were then co-stained with MAP-2 (blue). Graph shows quantification of mean fluorescence intensity (arbitrary units) in soma quantified from 4 independent experiments with 8–12 neurons per condition using ImageJ. B) Representative examples of dendritic regions of neurons overexpressing SENP-1 C603S (inactive control) or WT. Graph shows quantification of mean fluorescence intensity (arbitrary units) in dendrites. Quantification from 4 independent experiments; each experiment: 8–12 neurons per condition and 4 dendrites per neuron, done using ImageJ Scale bars – 5 µm. C) In situ hybridization with SUMO-1 negative control (sense probe) yielded virtually no signal compared to the anti-sense (experimental) probe. Scale bars – 20 µm. In all cases statistical significance was determined using ANOVA followed by Bonferroni post-hoc test (*p<0.01).