Horizontal Gene Transfer Contributed to the Evolution of Extracellular Surface Structures: The Freshwater Polyp Hydra Is Covered by a Complex Fibrous Cuticle Containing Glycosaminoglycans and Proteins of the PPOD and SWT (Sweet Tooth) Families
Figure 5
Immunofluorescence staining of Hydra polyps with anti-PPOD4 antibody.
Polyps were fixed with PFA (A-I) or Lawdowsky’s fixative (J–O), stained with anti-PPOD4 or anti-GFP antibody and examined in the confocal microscope. Schematic diagrams indicate the positions of the optical sections shown. Polyps in A-I were not permeabilized and show staining of the extracellular surface. Polyps in J-O were permeabilized to permit staining of intracellular vesicles. See text for details.