HTS-PEG: A Method for High Throughput Sequencing of the Paired-Ends of Genomic Libraries
Figure 2
The workflow of data processing.
The raw data were first filtered with the hairpin adaptor; reads without hairpin adaptor sequences were discarded. Then the vector sequences of the remaining reads were trimmed. The reads were then divided into left and right ends, and those reads with either end length less than 40 nt were discarded. The filtered paired-end reads were clustered and mapped to the genome.