HTS-PEG: A Method for High Throughput Sequencing of the Paired-Ends of Genomic Libraries
Figure 1
Flow chart illustrating HTS-PEG.
The plasmids of the genomic library were sheared to yield fragments of 100–1500 bp larger than the vector (Blue). Then, the EcoR I sites were methylated and hairpin adaptors (Red) which contain non-methylated EcoR I sites were ligated to the fragment ends. After EcoR I digestion and circularization, the paired ends can be amplified by primers that are complementary to the ends of the vector. The PCR products with the desired size can be sequenced using the high-throughput sequencing method.