Class I and Class II Histone Deacetylases Are Potential Therapeutic Targets for Treating Pancreatic Cancer
Figure 5
Synergistic interactions in inducing cell death between MGCD0103 and MC1568 or Tubastatin A in BxPC-3 or PANC-1 cells.
Three hundred PANC-1 cells or five hundred BxPC-3 cells were plated in 100 mm dishes 1 day prior to the treatments with variable concentrations of MGCD0103, MC1568, or Tubastatin A, alone (panels A and B) or in combination (panels C and D) for 96 h. The drugs were then washed out, and the cells were cultured in drug-free complete medium for up to 3 weeks. Colonies were visualized by coomassie blue staining and counted. Results are presented as mean percentages ± standard errors relative to untreated control cells from three independent experiments. * indicates p<0.05, while ** indicates p<0.005.