Flagella from Five Cronobacter Species Induce Pro-Inflammatory Cytokines in Macrophage Derivatives from Human Monocytes
Figure 7
Transfection assay in HEK293-hTLR5 cells enables IL-8 secretion in response to C. sakazakii flagella and flagellin.
293-hTLR5 cells (HEK293 cells transfected with TLR5) secreted 197 pg/ml and 133 pg/ml of IL-8 after treatment with C. sakazakii (ST1) flagella and flagellin, respectively; and 196 pg/ml of IL-8 were released with FliC-Salmonella enterica serovar Typhimurium. 293-hTLR5 cells pre-incubated with anti-hTLR5-IgA antibodies at a concentration of 10 µg/ml showed a reduction of 76% in IL-8 release.