Flagella from Five Cronobacter Species Induce Pro-Inflammatory Cytokines in Macrophage Derivatives from Human Monocytes
Figure 6
Anti-human-TLR5-IgA antibody blocked the secretion of pro-inflammatory cytokines.
(A) An inhibition dependent on concentration of anti-hTLR5-IgA was observed in IL-8 release, in flagella (51, 55, and 59%) and flagellin (21, 52 and 53%) with 1, 10 and 20 µg of antibody, respectively. (B) TNF-α release was blocked between 75–97% for flagella and 13–58% for flagellin with 1 and 10 µg anti-human-IgA-TLR5, respectively. (C) IL-10 release was blocked 98% for flagella and 99% for flagellin with 1 and 10 µg anti-human-IgA-TLR5, respectively. RPMI-1640 medium.