Flagella from Five Cronobacter Species Induce Pro-Inflammatory Cytokines in Macrophage Derivatives from Human Monocytes
Figure 5
Anti-flagella antibodies block the secretion of pro-inflammatory cytokines.
Purified flagella (100 pg/ml) and flagellin (100 ng/ml) were pre-incubated with anti-flagella antibodies in LPS-free water before the addition to macrophage cells. The antibodies were tested undiluted and using different dilutions (1∶10, 1∶100, and 1∶200). (A) IL-8 release was inhibited when flagella (94, 88, 48 and 48%) and flagellin (96, 94, 52 and 51%) were incubated with different dilutions of anti-flagella. (B and C) TNF-α and IL-10 releases were blocked completely with the three dilutions and with the undiluted antibody. RPMI-1640 medium.