Flagella from Five Cronobacter Species Induce Pro-Inflammatory Cytokines in Macrophage Derivatives from Human Monocytes
Figure 2
Biochemical analysis of flagella.
(A) Depolymerization of purified flagella in 12% SDS-PAGE gel. (B) Immunoblot assays with anti-flagella antibody recognizing the flagellin of ∼28-kDa. (C) Analysis of the protein band of ∼28-kDa by mass spectrometry. Molecular weight (MW).