Neuronal Basis of Innate Olfactory Attraction to Ethanol in Drosophila
Figure 5
Neuronal activity is required for preference.
After activation of UAS-Kir2.1 under the control of Tdc2-GAL4, tub-GAL80ts preference is significantly reduced (PIs are Tdc2-GAL4, tub-GAL80ts; UAS-Kir2.1 0.4±0.1 and with HS 0.12±0.09. n 22. P<0.05). Activation of a UAS-mCD8::GFP construct does not interfere with preference (PIs are Tdc2-GAL4, tub-GAL80ts; UAS-mCD8::GFP 0.22±0.16 and with HS 0.54±0.17. n 14.). Transgene insertions do not alter preference of flies carrying UAS-Kir2.2 and tub-GAL80ts (PIs are w1118; tub-GAL80ts; UAS-Kir2.1 0.29±0.11 and with HS 0.37±0.09. n 27 and 28.).