Neuronal Basis of Innate Olfactory Attraction to Ethanol in Drosophila
Figure 4
TbH is required in a subset of octopamingergic neurons for ethanol preference.
a Expression of TbH in a Tdc2-GAL4; Cha-GAL80 dependent manner in TbhnM18 mutants does not restore preference (PIs are Tdc2-GAL4/+; Cha-GAL80/+: 0.26±0.05, TbhnM18; Tdc2-GAL4; Cha-GAL80/+0.23±0.08 and TbhnM18, UAS-Tbh; Tdc2-GAL4; Cha-GAL80/+0.00±0.10, n 27, 22 and 25. The experimental group does not develop preference and differs significantly from the control. The transgene insertion in the mutant background differs significantly from respective control. P<0.05 and <0.001.). b Feb15-GAL4 dependent expression of TbH in TbhnM18 mutants restores preference (PIs are w1118; Feb15-GAL4/+0.37±0.07; w1118, TbhnM18; Feb15-GAL4/+0.14±0.07 and w1118, TbhnM18, UAS-Tbh; Feb15-GAL4/+0,47±0.04. n 28, 33 and 36. The level of preference of the experimental group does not differ from the control. The transgene insertion in the mutant background differs significantly from respective control. P<0.05.). c Loss of preference in TbhnM18 mutants is not completely restored by TbH expression under the control of the 6.2-Tbh-GAL4 driver (PIs are w1118; 6.2-Tbh-GAL4/+0.29±0.06, w1118, TbhnM18; 6.2-Tbh-GAL4/+0.06±0.08 and w1118, TbhnM18, UAS-Tbh; 6.2-Tbh-GAL4/+0.13±0.08. n 36, 26 and 36. The olfactory preferences of the control group and experimental group are not significantly different. Mutants carrying one copy of the GAL4 transgene differ significantly from the control carrying one copy of the GAL4 transgene. P<0.05.). The schemata below graphs for behavioral experiments summarize the expression domains of respective GAL4 drivers in comparison to TbH expression. White cells are TbH positive neurons not targeted by specific driver lines and grey cells indicate co-localization of GAL4 and TbH. The letter a labels an olfactory preference score that is significantly different from random choice as determined by One-sample sign test.