Use of the Foot-and-Mouth Disease Virus 2A Peptide Co-Expression System to Study Intracellular Protein Trafficking in Arabidopsis
Figure 5
Analysis of activity of SAR1 and ARF1originating from 2A constructs.
Representative confocal images of Arabidopsis protoplasts transiently expressing GS-CFP-2A-SAR1 (wt) (A and B), GS-CFP-2A-SAR1 (H74L) (C and D); GS-CFP-2A-ARF1 (wt) (E and F), GS-CFP-2A-ARF1 (T31N) (G and H) and GS-CFP-2A-ARF1 (Q71L) (I and J). Bars = 5 µm. (K) Analysis of fluorescent Golgi stacks size in Arabidopsis protoplasts transiently expressing GS-CFP-2A-ARF1 (wt) or mutant (Q71L) forms. Error bars show standard error (n = 14, *** = p<0.001).