Specific Micro RNA-Regulated TetR-KRAB Transcriptional Control of Transgene Expression in Viral Vector-Transduced Cells
Figure 5
Lentiviral vectors used to target transgene expression to skeletal muscle-derived cells.
(a) Schematic diagram of the lentivirus vectors used to accomplish skeletal muscle-specific expression. (b) miR-133 expression levels in C2C12d, C2C12ud and 293T cells detected by RT-qPCR. Three independent experiments were performed in duplicate. *Statistically significant differences P = 0.0185 (two-tailed, unpaired Student’s t-test). (c) Differentiated or undifferentiated C2C12 cells (C2C12d and C2C12ud respectively) were transduced with lentiviral vectors carrying the TetR-KRAB sequence followed by four copies of miRNA target of miR-122 or miR-133. After 10 days of culture with or without doxycycline, transduced cells were analyzed for GFP expression by FACS. Results are expressed as a percent of MFI. The raw data corresponding to 100% are indicated above each histogram bar. Data shown are mean and error bars indicate the SD of 3 independent experiments performed in triplicate.