Monitoring Intracellular Calcium Ion Dynamics in Hair Cell Populations with Fluo-4 AM
Figure 5
Blocking the transduction channel decreases hair-bundle and cell body Ca2+.
(a–d) Control hair cells have Fluo-4 signal in the bundle (b) and cell body (d). (e–h) Hair cells incubated with 100 µM tubocurarine to block the transduction channel have decreased Fluo-4 signal in the bundle (f) and cell body (h). (i–j) To quantify Fluo-4 fluorescence, individual bundle and cell body ROIs were selected and integrated density of Fluo-4 channel was calculated (see Figure 6), then normalized to the control mean for each group. (a, c, e, g) Corresponding DIC images. (au = arbitrary units; control bundles n = 63, tubocurarine bundles n = 73, control cell bodies n = 72, tubocurarine cell bodies n = 77; **** p<0.0001).