Activation Mechanisms of Natural Killer Cells during Influenza Virus Infection
Figure 5
Activation of STAT4 is partially dependent on the direct action of type I IFNs during influenza infection.
NK cells from infected (open histograms) and uninfected (shaded histograms) mice were analyzed for intracellular pSTAT4 following adoptive transfer (A) or co-culture (B). Adoptive transfer was as described in Figure 2. For in vitro infection, CD45.2+ splenocytes from IFNAR+/− or IFNAR−/− mice were combined with CD45.1+ B6 splenocytes at 1∶1 ratio, then infected with flu. NK cells (NK1.1+CD3−) from infected (open histograms) and uninfected (shaded histograms) samples were analyzed for intracellular pSTAT4. Values represent the percentages of pSTAT4+ NK cells. Data are representative of three independent experiments with 2–4 (A) or 1–3 (B) mice per group.