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Live-Cell Visualization of Intracellular Interaction between a Nuclear Migration Protein (hNUDC) and the Thrombopoietin Receptor (Mpl)

Figure 5

Western blot analysis of hNUDC domains in the medium of cultured Dami cells.

(A) Dami cells were transfected with Ad-hNUDC. (B) Dami cells were transfected with Ad-hNUDC(1-159). (C) Dami cells were transfected with Ad-hNUDC(160-331). Secreted hNUDC, hNUDC(1-159) or hNUDC(160-331) in the culture medium was assayed by immunoblot using an anti-hNUDC antibody at each time point indicated.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0051849.g005