ATM Suppresses SATB1-Induced Malignant Progression in Breast Epithelial Cells
Figure 5
The combination of ectopic SATB1 expression and ATM depletion induces aggressive phenotype in non-malignant mammary epithelial cells.
A) Immunoblot showing ATM and SATB1 levels before and after SATB1 overexpression (SATB1) and ATM depletion (shATM) in non-malignant MCF10A-1, MCF10A-2, 184A1 and 184B5 cells. α-tubulin was used as an internal loading control. B) Colony morphologies of vector control, ATM depleted (shATM), SATB1 overexpressing (SATB1) and ATM depleted/SATB1 overexpressing (shATM+SATB1) cells generated as in (C). Cells were grown in 3D Matrix on-top cultures. The images were captured with phase 1 at 20X magnification on five days after plating. Scale bars, 100 µm. D) Invasion assay of ATM depleted (shATM), SATB1 overexpressing (SATB1) and ATM depleted/SATB1 overexpressing (shATM+SATB1) cells generated as in (a). Error bars indicate ±s.e.m., n = 3 experiments.