H3K56me3 Is a Novel, Conserved Heterochromatic Mark That Largely but Not Completely Overlaps with H3K9me3 in Both Regulation and Localization
Figure 5
H3K56me3 is conserved in Caenorhabditis elegans.
Shown are representative IF microscopy pictures from adult C. elegans hermaphrodite tissues. In all images H3K56me3 is shown in green, H3K9me3 in red, and DAPI (DNA) in blue. Scale bar = 5 µm. A) H3K56me3 co-localizes with H3K9me3 in the early germline, late pachytene and in a 100-cell embryo (top picture). Interestingly, although H3K56me3 and H3K9me3 are both present in oocytes, only H3K56me3, but not H3K9me3, staining could be observed in sperm. (bottom, split channels) (B) H3K56me3 and H3K9me3 co-localize throughout all stages of mitosis.