Regulation of Cell Wall-Bound Invertase in Pepper Leaves by Xanthomonas campestris pv. vesicatoria Type Three Effectors
Figure 6
Screening for Xcv T3Es involved in regulation of cw-Inv activity.
Leaves of pepper plants were infiltrated with wild type and mutant Xcv strains at 109 cfu ml−1 and cw-Inv activity was measured 2 and 3 days post infection (dpi) in independent experiments. Graphs represent values calculated relative to the Xcv wild type (wt) response which was set to 100% for each individual experiment. Mean cw-Inv activities after infection with Xcv wild type were 20.96 µmol min−1 m−2±8.43 (100% ±40.2) and 57.57 µmol min−1 m−2±23.92 (100% ±41.6) at 2 and 3 dpi, respectively. The variance of Xcv wild type response (ca. 42%) is illustrated as a dashed line. Values are the mean response (as percentage to Xcv wild type) ± SD from three to nine different experiments. Statistically significant differences from Xcv wild type response were determined using two-tailed t-test assuming normal distribution and are indicated by asterisks (**p<0.01); (***p<0.001).