Ascl1 (Mash1) Knockout Perturbs Differentiation of Nonneuronal Cells in Olfactory Epithelium
Figure 5
HBC Emergence is aberrant but not aborted completely as a consequence of Ascl1 KO.
Matched sections from E19.5 HET and Ascl1 KO nasal epithelium were stained for K14 to mark HBCs and βIV TUBULIN to mark respiratory epithelium. K14 staining was highlighted and cartooned in black and βIV TUBULIN was highlighted and cartooned in red. Representative sections were arranged from Rostral to Caudal levels (top to bottom, matched levels numbered 1 to 5). Coincident black and red indicate regions of respiratory epithelium that contain a dense population of K14 (+) basal cells, while areas of black only represent the emergence of K14(+) HBCs in olfactory epithelium. In the HET (left side) HBCs are relatively abundant in the caudal olfactory areas, while few if any are seen in comparable regions of the OE in the Ascl1 KO (right side).