Accurate Construction of Photoactivated Localization Microscopy (PALM) Images for Quantitative Measurements
Figure 5
Accuracy of images generated with different threshold pairs.
(A) Region of threshold space (white squares) that resulted in <10% difference from the reference measurements of Z-ring width, N, and fmidcell, and that yielded Z-ring density distributions not significantly different from the reference distribution (pKS >0.05). (B) Jaccard index values at each threshold pair. Higher Jaccard index values indicate more accurate single-molecule clustering. (C) The peak of the Jaccard index plot (B, white squares) is within the region where all four quantitative measurements are within 10% of the reference measurements (A). Dataset analyzed is the same simulated dataset shown in Figure 2–4.