Most Anti-BrdU Antibodies React with 2′-Deoxy-5-Ethynyluridine — The Method for the Effective Suppression of This Cross-Reactivity
Figure 5
The increase of azido dye concentration and acid treatment results in a non-specific signal.
The picture shows examples of the detection of incorporated EdU in DNA by means of a click reaction with 0.02 mM fluorescent dye without the HCl treatment (images labelled as control) and of detection with 0.2 mM fluorescent azido dyes and the antibody clone BU1/75 (Cy3 anti-rat and FITC anti-rat secondary antibodies) in cells treated with 4N HCl. Note that the higher concentration of fluorescent azido dyes and the subsequent treatment with 4N HCl led to a non-specific signal mainly in the nucleolus area. Barr: 20 µm.