Dendritic Cells from Oral Cavity Induce Foxp3+ Regulatory T Cells upon Antigen Stimulation
Figure 5
Phenotype of dendritic cells from cervical lymph nodes.
(A) DCs from CLN, ALN, and MLN were freshly prepared from B6 mice. mRNA was prepared and real-time PCR was performed. Expression of each sample was normalized to GAPDH mRNA expression and fold increase of each sample was calculated relative to the expression at 0 h. One of two separate experiments is shown. (B) DCs from CLN, ALN, and MLN were analyzed for the expression of CD103. The plots were gated on CD11c+ cells. The isotype control for CD103 is shown at the bottom. The graphic shows a summary of four separate experiments. Average +/− SD is shown. (C) As in (B), DCs from CLN, ALN, and MLN were analyzed for the expression of MHC class II or B220. The plots were gated on CD11c+ cells. The graphic shows a summary of four separate experiments. Average +/− SD is shown. (D) As in (B), DCs from CLN, ALN, and MLN were analyzed for the expression of CD8. The plots were gated on CD11c+ cells. The graphic shows a summary of 10 separate experiments. P value provided is by paired t-test. “n.s.” = “not significant”.