Novel Potential Interacting Partners of Fibronectin in Spontaneous Animal Model of Interstitial Cystitis
Figure 5
Immunohistochemical double labelling of candidates in healthy (left panels) and diseased bladder tissue (right panels).
Immunohistochemical double staining of a healthy urinary bladder (A) shows considerable colocalization of fibronectin (green) and its interactor thioredoxin (red) in the subepithelial and muscular tunices. In contrast, lack of green and red colour is evident in FIC (B), indicating a loss of both fibronectin and thioredoxin from its normal distribution in healthy bladder tissue. Overlay image of thioredoxin (red) and NF-κB p65 (green) in a healthy bladder tissue (C) shows a predominant signal of thioredoxin in all tunices of the bladder, whereas NF-κB p65 is not detectable in any tunic of the healthy bladder. In contrast, thioredoxin and NF-κB p65 colocalize (overlapping results in yellow colour) in the lamina propria mucosae with the highest expression in the extracellular matrix of FIC diseased bladder tissue (D). P38 MAPK signal is of moderate intensity localized in transitional epithelium cells and around few blood vessels in the healthy bladder tissue (E). Note that the signal is exclusively of yellow colour indicating a colocalization with thioredoxin, whereas a green colour signal is not visible at all. In contrast, p38 MAPK (green) was highly expressed in the cytoplasm of umbrella cells of the transitional cell epithelium as well as a scattered expression around cell nuclei in the subepithelial and muscular tunices of FIC diseased bladder sections without distinct colocalization (F). The blue colour reveals staining of cell nuclei (DAPI). a = Transitional cell epithelium, b = Lamina propria mucosae, c = Muscle tunic.