Inner Ear Morphology Is Perturbed in Two Novel Mouse Models of Recessive Deafness
Figure 2
Myo7a mutations identified in Myo7aI487N/I487N ewaso and Myo7aF947I/F947I dumbo strains.
(A) Direct sequencing identified a 1460T>A (I487N) missense mutation in Myo7aI487N/I487N ewaso and a missense mutation 2839T>A (F947I) in the Myo7aF947I/F947I dumbo mouse strain (B). Wildtype and heterozygote sequences are shown for comparison. (C) Sequence conservations of Myo7aI487N/I487N ewaso (red) and Myo7aF947I/F947I dumbo (blue) mutations, and the closely positioned Myo7ash-1 shaker mutation (green) showing high evolutionary conservation of our two mutant strains.