Atbf1 Regulates Pubertal Mammary Gland Development Likely by Inhibiting the Pro-Proliferative Function of Estrogen-ER Signaling
Figure 5
Cell proliferation activity is increased by the knockout of Atbf1 in mammary epithelial cells from 6-week old mice.
(A–B) Tissue sections of mammary ducts (A) and terminal end buds (TEBs) (B) were subjected to IF staining against Ki67. DAPI staining was used to show nuclei (blue) and Ki67 was labeled by RITC (red). (C) Ratios of Ki67-positive cells to total epithelial cells in mammary glands with different Atbf1 genotypes were calculated. Each bar indicates the average result from 10 randomly selected areas. Three mice were used for each genotype, and the number of mammary epithelial cells counted for each bar was 1200–1600 for ducts and 2000–2800 for TEBs. *, P<0.05 and **, P<0.01 (one-way ANOVA).