Regulation of per and cry Genes Reveals a Central Role for the D-Box Enhancer in Light-Dependent Gene Expression
Figure 5
Light induced D-box enhancer activity requires de novo protein synthesis.
(A–C and E) qRT-PCR analysis of luciferase mRNA expression in PAC-2 cells transfected with different heterologous luciferase reporter constructs, in the presence (red traces) or absence (green traces) of CHX during 8 hours of light exposure or DD conditions (+CHX, blue traces, −CHX, black traces). (D) qRT-PCR analysis of endogenous per1b expression in PAC-2 cells in the presence or absence of CHX during 8 hours of light exposure or DD conditions (colour coded the same as in panels A–C and E). Each construct is indicated above its respective panel. Relative mRNA levels are plotted on the y-axis and were set arbitrarily as 1 at time-point 0 hrs. Time (hrs) is plotted on the x-axis. In each panel, points are plotted as means of three independent experiments +/− SD. All statistical analyses (two-way ANOVA) are presented in Table S2 B or in the results section.