Novel Interactions between FOXM1 and CDC25A Regulate the Cell Cycle
Figure 2
The FOXM1 transcription factor regulates CDC25A gene expression.
(A) FOXM1 increased the transactivation of CDC25A promoter. U2OS cells were co-transfected with pGL3-CDC25A promoter reporter along with increasing doses of pACT-FOXM1 for 48 h. pACT vector was used as the empty vector, and pRL-SV40 expressing Renilla luciferase was used as an internal control. Data represent the mean ± SD (N = 3). *, p<0.05; ***, p<0.001. (B) siRNA-mediated silencing of FOXM1 decreased CDC25A expression. U2OS cells were transiently transfected with siRNA pools targeting FOXM1 or non-target control for 48 h. A U2OS non-transfected control was also used. The protein expression of FOXM1 and CDC25A were detected by Western blot. β-Actin expression was used as a loading control. The western blot was repeated three times and the protein expression was analyzed by the Adobe Photoshop CS4 software (Adobe Systems, San Jose, CA). The difference of band density between the FOXM1-siRNA and control siRNA was analyzed by the paired t test. ***, p<0.001.