Label-free Evaluation of Hepatic Microvesicular Steatosis with Multimodal Coherent Anti-Stokes Raman Scattering Microscopy
Figure 5
Visualization and characterization of lipid-rich non-parenchymal cells (NPCs) with CARS and TPF imaging and spontaneous Raman microspectrometry analysis.
(A) CARS imaging of a lipid-rich NPC (arrow) in the interstitial space of hepatocytes. (B) CARS and TPF imaging of lipid-rich and autofluorescent lipid-rich NPCs. (C) Comparison of representative Raman spectra of hepatocytes (blue) and lipid-rich NPCs (red). Note the distinctive Raman peak at 1620 cm−1 for lipid-rich NPCs.