Identification of Regions Involved in Substrate Binding and Dimer Stabilization within the Central Domains of Yeast Hsp40 Sis1
Figure 2
Functional tests of wild-type Sis1 and mutants and Ydj1.
The ability of yeast DnaJ and mutants in binding to Hsp70 Ssa1 (A) and in cooperating with Hsp70/Ssa1 to refold heat-denatured firefly luciferase (B) was monitored. Sis1 activity was set as a standard (100%).