Screening the Budding Yeast Genome Reveals Unique Factors Affecting K2 Toxin Susceptibility
Figure 2
Distribution of cellular processes and cellular components involved in K2 resistance and hypersensitivity.
A. Agar diffusion assays. Representative examples of “halo” observed for strains with different degree of sensitivity/resistance towards K2 toxin (see Figure 1B, screen 5, for details). An isogenic control (wt, strain BY4741) is provided in the center. Strains Δkre1, Δchw41, and Δmrpl3 are resistant (show a smaller “halo” than wild-type or no “halo”). Strains Δrpl14a, Δspt8, and Δhog1 are hypersensitive (show a larger “halo” than wild-type). B. Distribution of cellular pathways and cellular components, according to Gene Ontology, associated with the 205 genes associated with K2 resistance and 127 with K2 hypersensitivity. The number of genes identified in each class is indicated.