UV-Light Exposure of Insulin: Pharmaceutical Implications upon Covalent Insulin Dityrosine Dimerization and Disulphide Bond Photolysis
Figure 11
Effect of continuous 276 nm light exposure of human insulin on its hormonal function in vitro.
The glucose uptake by human skeletal muscle cells was measured after 1 hour of incubation at three different conditions: no insulin (control), insulin kept in the dark (positive control, PC), and UV-illuminated insulin (1.5 h exc. at 276 nm). Uncertainty values (standard errors) for the concentration of glucose (uptake) are displayed with error bars. The students t-tests resulted in the following p-values: p (control vs PC) = 27.6.10−4; p (control vs 1.5 h exc. at 276 nm) = 0.002165; p(PC vs 1.5 h exc. at 276 nm) = 0.00179.