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UV-Light Exposure of Insulin: Pharmaceutical Implications upon Covalent Insulin Dityrosine Dimerization and Disulphide Bond Photolysis

Figure 7

Fluorescence excitation spectra of human insulin (emission fixed at 405 nm) upon prolonged 276 nm UV-excitation.

Fluorescence excitation spectra (emission fixed at 405 nm) obtained before and after 276 nm light continuous exc. (0.5 h, 1 h, 1.5 h, 2.5 h, 3.5 h, and 7 h) of human insulin in solution. There is a continuous increase in excitation intensity at 320 nm with 276 nm exc. time.

Figure 7

doi: https://doi.org/10.1371/journal.pone.0050733.g007