Methylselenol Formed by Spontaneous Methylation of Selenide Is a Superior Selenium Substrate to the Thioredoxin and Glutaredoxin Systems
Figure 4
Inhibition of thioredoxin mediated protein disulfide reductase activity.
The method was performed as described by Kumar et al. [6]. The reaction mixture contained 80 mM HEPES buffer, pH 7.6, 3 mM EDTA and 0.7 mM NADPH. TrxR1 and Trx1 were added to a final concentration of 8 nM and 1 µM respectively. The measurements were performed with the following selenite concentrations (1, 5 and 10 µM). The amount of SH-group formed was measured at 412 nm. Grey bars: Addition of selenite at varying concentrations. White bars: Addition of both selenite and SAM (4 mM). Student t-test, dependent by samples, was used for statistical analysis (*p<0.05, **p<0.01).