Cardiac Ankyrin Repeat Protein Attenuates Cardiac Hypertrophy by Inhibition of ERK1/2 and TGF-β Signaling Pathways
Figure 5
Upregulated TGF-β/Smad signaling is inhibited in CARP Tg mice following TAC.
(A) Quantitative detection of TGF-β1, -β2, and -β3 mRNA expression by real-time PCR in hearts from WT and CARP Tg mice subjected to TAC or a sham operation. (B) ELISA measurements of TGF-β1 levels in heart homogenates from WT and CARP Tg mice subjected to TAC or a sham operation. (C) Western blotting to detect phosphorylated and total Smad3 protein in heart protein extracts from WT and CARP Tg mice subjected to TAC or a sham operation. (D) Quantification of the level of phosphorylated Smad 3 shown in (C).