Breast Cancer Stem Cell-Like Cells Are More Sensitive to Ionizing Radiation than Non-Stem Cells: Role of ATM
Figure 3
Flow cytometry analysis of cell cycle in asynchronous and effect of aphidicolin treatment on phase distribution and radiosensitivity in non-stem and CSC-like cells.
Flow cytometry was performed on non-stem and stem-like MDA-MB-453 cells (A) or MDA-MB-231 cells (B). The percentages of cells in the G2/M, S, and G1 were analyzed and plotted. Non-stem and CSC-like MDA-MB-453 cells were treated with aphidicolin (5 µM) for 16 hr. After aphidicolin treatment, cell cycle was analyzed (C) and radiation sensitivity was determined by colony formation after irradiation (6.25 Gy) (D). Error bars represent standard error from the mean for three separate experiments.