Lipopolysaccharide-Induced Expression of Microsomal Prostaglandin E Synthase-1 Mediates Late-Phase PGE2 Production in Bone Marrow Derived Macrophages
Figure 8
The enzyme activities of expressed COX-2 and mPGES-1 enzymes between early and late phase of LPS treatment were not different.
The induced COX-2 and mPGES-1 enzymes were immunoprecipitated (IP, 2 hrs at 4°C) separately from equal amount of BMDM lysates at either 8 or 16 hrs of LPS treatment under the same experimental condition. The IP COX-2 or mPGES-1 enzyme concentrations were determined, and equal amount of IP COX-2 or mPGES-1 enzymes from each time point was used to determine their enzyme activity of PGE2 production from their substrates (either arachidonic acid or PGH2) in vitro. No significant difference in either COX-2 or mPGES-1 enzyme activity of PGE2 production was detected between 8 and 16 hrs of LPS treatment (n = 3).