Lipopolysaccharide-Induced Expression of Microsomal Prostaglandin E Synthase-1 Mediates Late-Phase PGE2 Production in Bone Marrow Derived Macrophages
Figure 3
LPS stimulates PGE2 and PGD2 production in BMDM.
Equal amount of BMDM was treated with or without 1 µg/ml LPS for various time points from 2 to 24 hrs. The BMDM culture medium was collected and the concentrations of PGE2 and PGD2 in the sample media were quantified using LC-MS-MS. LPS induced both PGE2 and PGD2 production at a similar lower level in the first 8 hrs of treatment. In contrast, the production of PGE2 in BMDM continuously increased after LPS treatment for 12 hrs; whereas the production of PGD2 kept at a relatively stable level after 12 hrs of LPS treatment (n = 5).