Multiple-Level Regulation of 2,4-Diacetylphloroglucinol Production by the Sigma Regulator PsrA in Pseudomonas fluorescens 2P24
Figure 5
RpoS regulates the 2,4-DAPG production via RsmA in P. fluorescens 2P24.
Biosynthesis of 2,4-DAPG in strains 2P24 and its rpoS mutant was assayed by HPLC (A). The expression of the rpoS gene is activated by PsrA in strain 2P24 (B). Expression of the rsmA gene in the wild type strain 2P24 and the rpoS mutant PM303 (C). Binding assay of PsrA to the rpoS promoter. 30 ng DNA probe was incubated with increasing amounts of PsrA. Lane 1, DNA probe alone; lanes 2–5, DNA probe incubated with 25, 50, 75, or 100 ng PsrA, respectively; lane 6, the mutated DNA probe from p399rpoSp derivative (a 3-bp substitution [GGG for TTT] in the rpoS promoter) incubated with 100 ng PsrA (D). Western blot analysis of RsmA-V in strain 2P24 and the rpoS mutant (E).