Ataxin-2-Like Is a Regulator of Stress Granules and Processing Bodies
Figure 5
A reduced ATXN2L level affects the size and number of SGs.
HeLa cells were left untreated (mock) or transfected with siRNAs against ATXN2L or ATXN2 transcripts, or non-targeting control siRNA (siNT). 72 hours post transfection cells were treated with 0.5 mM sodium arsenite for 1 hour and fixed. A) For the confocal microscopy proteins were stained with an antibody detecting eIF4G. (B-D) For the quantitative high-content screening microscopy staining occurred with antibodies detecting eIF4G and TIAR. B) Cell number and size of nuclei was analyzed by quantitative high-content screening microscopy. C, D) Quantification of TIAR- and eIF4G-positive SGs regarding number and size. Results are expressed as mean ± SD from one representative experiment, n = 5 replicate wells, *p<0.05; **p<0.01; ***p<0.001, One-way ANOVA with Tukey’s Multiple Comparison post test.