PPAR-γ Activation Increases Insulin Secretion through the Up-regulation of the Free Fatty Acid Receptor GPR40 in Pancreatic β-Cells
Figure 2
RGZ induces intracellular calcium mobilization from extra- and intra-cellular sources.
(A) Effects of nifedipine (10 mM), thapsigargin (0.1 µM), and GLUT2 sequence-specific silencing with RNAi on 10 µM RGZ-induced intracellular calcium mobilization and (B) insulin secretion (ANOVA within same glucose conditions: n = 4, * P<0.01 vs. RGZ treatment with 5.6 mM glucose; **P<0.05 vs. non-treatment with 16.7 mM glucose; and ***P<0.01 vs. RGZ treatment with 16.7 mM glucose) (C) Immunoblot for GLUT2 expression with 10 µM RGZ treatment and/or adenoviral PPAR-γ overexpression.