Remarkable Diversity in the Enzymes Catalyzing the Last Step in Synthesis of the Pimelate Moiety of Biotin
Figure 6
The BioG proteins cleave the ester group of pimeloyl-ACP methyl ester.
The reaction mixtures containing pimeloyl-ACP methyl ester were mixed with purified BioH as a positive control or a purified BioG from one of four different bacteria as shown. Lane 1 lacked enzyme added. Following incubation for 1 h 10 µl of the reaction mixture was analyzed by electrophoresis on a 20% polyacrylamide gel containing 2.5 M urea.