Remarkable Diversity in the Enzymes Catalyzing the Last Step in Synthesis of the Pimelate Moiety of Biotin
Figure 5
Purification of His6-tagged BioG proteins.
Samples (10 µl) of each eluted fraction were analyzed by electrophoresis on 10% SDS-polyacrylamide gels. The lysate and soluble fractions are given in lanes L and S, respectively. The protein was eluted from the Ni-NTA column with a buffer containing 200 mM imidazole. The fractions shown were pooled and dialyzed as described in Experimental Procedures. Low range molecular weight markers are shown in lanes marked M.