Remarkable Diversity in the Enzymes Catalyzing the Last Step in Synthesis of the Pimelate Moiety of Biotin
Figure 3
Sequence alignments putative biotin synthetic esterases show conserved catalytic triad residues.
Homologues of BioH, BioG and another putative isozymes, BioK, were obtained from the SEED database (http://theseed.uchicago.edu/FIG/index.cgi). Shown are some of the sequences from a MUSCLE [18] alignment with a −1 extension gap penalty. The putative catalytic sites are shaded in yellow. The residue numbers (given in Italics) are those of E. coli BioH, H. influenzae BioG and P. marinus BioK.