Plasmodium falciparum UvrD Helicase Translocates in 3′ to 5′ Direction, Colocalizes with MLH and Modulates Its Activity through Physical Interaction
Figure 13
Models for PfMLH-stimulated unwinding. A,
Using normal partially duplex circular substrate (substrate 1, Tale S1). 1. Incubation with PfMLH. 2. It creates non-specific nicks. 3. It results in unstable substrate, which melts at 37°C and addition of PfUDN has no effect. B, Using direction-specific substrate (substrate 3B, Tale S1). 1. Incubation with PfMLH results in its binding to substrate. 2. Incubation with PfUDN facilitates its loading to the substrate through PfMLH. 3. It results in stimulation of unwinding in 3′ to 5′ direction.