Plasmodium falciparum UvrD Helicase Translocates in 3′ to 5′ Direction, Colocalizes with MLH and Modulates Its Activity through Physical Interaction
Figure 10
Protein-protein interaction study. A,
ELISA based protein-protein interaction study of PfMLH with PfUDN, PfUDC1, and PfUDC2. The proteins coated such as PfUDC1, PfUDC2, PfMLH and PfUDN are written in the first column followed by the interacting proteins. The interaction was done as reported in materials and methods section. This experiment was repeated at least three times and the quantitative data are shown. B, Western blots of protein-protein interaction. Lane 1 and 2 are eluted fractions of in vitro immunoprecipitates of anti PfUDN IgG protein A sepharose column, lane 3 is immunoprecipitate of preimmune IgG protein A sepharose column. Lane 1 is probed with anti-PfUDN antibody; lanes 2 and 3 were probed with anti-MLH antibody.