Plasmodium falciparum UvrD Helicase Translocates in 3′ to 5′ Direction, Colocalizes with MLH and Modulates Its Activity through Physical Interaction
Figure 5
Immunodepletion of ATPase and helicase activities.
A and B, Quantitative data of immunodepletion of ATPase activity. A, Lanes 1–3, reactions with increasing concentration of PfUDN pretreated with immune IgG and lanes 4–6, reactions with increasing concentration of PfUDN pretreated with pre-immune IgG. B, Lanes 1–3, reactions with increasing concentration of PfUDC1 pretreated with anti-his IgG and lanes 4–6, reactions with increasing concentration of PfUDC1 pretreated with pre-immune IgG. Each bar represents the mean percentage ± SD of two different experiments. C and D, Quantitative data of immunodepletion of helicase activity. C, Lanes 1–3, reactions with increasing concentration of PfUDN pretreated with immune IgG and lanes 4–6, reactions with increasing concentration of PfUDN pretreated with pre-immune IgG. D, Lanes 1–3, reactions with increasing concentration of PfUDC1 pretreated with anti-his IgG and lanes 4–6, reactions with increasing concentration of PfUDC1 pretreated with pre-immune IgG. Each bar represents the mean percentage ± SD of two different experiments.